Cell Signaling Anti Lamin

Anti lamin a c antibodies are available from several suppliers.

Cell signaling anti lamin.

Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation. Lamin a is a reported synonym of the human protein lamin a c encoded by the gene lmna. Phosphorylation of lamin a c at ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens. In humans this protein is encoded by the gene lmna.

The cells were fixed in 2 paraformaldehyde for 10 min at room temperature. 2000 j struct biol 129 313 23. Lamin b1 is also cleaved by caspases during apoptosis 9. Find out why customers rank cst highest for antibody specificity and sensitivity.

Lamin b1 is also cleaved by caspases during apoptosis 9. Anti lamin a antibodies are readily available from several suppliers. Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation. Type b lamins consist of lamin b1 and b2 encoded by separate genes 6 8.

The cleavage of lamins results in nuclear dysregulation and cell death 5 6. The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a. 2000 j struct biol 129 313 23. Incubate membrane with anti rabbit igg hrp linked antibody 7074 at 1 2000 and anti biotin hrp linked antibody 7075 at 1 1000 1 3000 to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.

Antibodies used were polyclonal goat anti lamin b 6216 santa cruz biotechnology dallas tx usa. The cleavage of lamins results in nuclear dysregulation and cell death 5 6. The full protein is reported to be 664 amino acid residues in length. Wash three times for 5 min each with 15 ml of tbst.

Type b lamins consist of lamin b1 and b2 encoded by separate genes 6 8. During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4. Actin filaments were labeled by dylight 554 phalloidin 13054 cell signaling technologies danvers ma usa. During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.

The expected protein mass is 74 1 kda but there are 6 reported isoforms. The cleavage of lamins results in nuclear dysregulation and cell death 5 6. Proceed with detection section d. Lamin a and c are cleaved by caspases into large 41 50 kda and small 28 kda fragments which can be used as markers for apoptosis 4 5.

An antibody shouldn t be one of the variables in your experiment.